HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2)

HAPLN4 (hyaluronan and proteoglycan link protein 4; also Bral2) is a 40-42kD secreted member of the Link module superfamily. It is secreted by select neurons, particularly of the cerebellum, where it participates in ECM stability. Here it may link hyaluronic acid with resident proteoglycans. Mature HAPLN4 is 383 amino acids (aa) in length. It contains one lg-like C2-type domain (aa 46-161) and two link domains (aa 163-365). One potential alternate start site exists 36 aa upstream of the standard start site. Human and mouse HAPLN4 share 91% aa sequence identity.\n\nApplications: \nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 10 ng/lane under non-reducing and reducing conditions. \nImmunoprecipitation: This antibody has been used to immunoprecipitate rhHAPLN4 from conditioned media of transfected NS0 cells.\nImmunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect HAPLN4 on SHSY5Y human neuroblastoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degrees C followed by NorthernLightsTM 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. \nOptimal dilutions should be deterhined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1818-50B

Size

100ug

Applications

ICC, IP, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human HAPLN4 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human HAPLN4 in the applications listed. In Western Blots, this antibody shows less than 1% crossreactivity with rhHAPLN1.