Haptoglobin, Human (HP, BP, HP2-ALPHA-2, HPA1S, MGC111141) BioAssay(TM) ELISA Kit

Acute phase proteins are plasma proteins which increase in concentration following infection,\ninflammation or trauma. The first acute phase proein to be recognized was discovered in humans by Tillet and Frances in 1930 (1). Haptoglobin (Hp) is a heterogeneous plasma protein mostly synthesized by the liver. The haptoglobin monomer consist of two heavy chains, beta chains (40kD) and two light chains, alpha chains, alpha 1 (9kD) and alpha 2 (16kD) that are linked disulfide bonds. The three major haptoglobin types are; Hp1-1 which is monomeric (98kD), Hp1-2 is polymeric at about 200kD, and Hp2-2 at about 400kD. The levels in serum rise quickly following acute tissue damage within 24 to 48 hours and also fall very rapidly once the stimulus is removed. In fact, Hp level are decreased in hemolytic anemia. Hp has a high affinity for hemoglobin (Hb) and its function appears to be to prevent loss of Hb in urine which would lead to loss of iron. Investigations over the past few years have shown that quantification of Hp in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well (2).\n\nSample Type:\nSerum/Plasma\n\nIntended Use:\nThe Haptoglobin test kits are a highly sensitive twosite enzyme linked immunoassay (ELISA) for\nmeasuring Haptoglobin in serum or plasma of Human. Crossreactivity with Cynomolgus Monkeys has been observed.\n\nTest Principle:\nThe principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the\nHaptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3

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SPECIFICATIONS

Catalog Number

H1820-04J

Size

1Kit

References

1. Tillett, W.S. and T. Francis. 1930. Serological reactions in pneumonia with non-protein somatic\nfraction of pneumococcus. J. Exp Med. 52:561-571. 2. Eckersal, P.D. 2000. Recent advances and\nfuture prospects for the use of acute phase proteins and markers of disease in animals. Revue Med. Vet.\n151(7): 577-584.