Histone acetyltransferases (HATs) have been implicated in a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Sample Type:
Cell and tissue lysates.
Intended Use:
Detects HAT activity by colorimetric method in mammalian samples.
Test Principle:
HAT Activity Colorimetric BioAssay(TM) Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Kit Components:
HAT buffer, 2x7.5ml
HAT Substrate I, 1x vial
HAT Substrate II, 1x vial
NADH Generating Enzyme, 1x800ul
Nuclear Extract (NE), 4mg/ml, 1x50ul
HAT Reconstitution Buffer, 1x1.8ml
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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