HAT Assay Reagent Kit, BioAssay(TM) (Histone Acetyl Transferase)

Kit Description:\nQuantity: 100 assays per kit\nUse: Accessory reagent kit for the analysis of histone acetyltransferase activity.\n\nKit Components:\nH1823-01A: HAT Assay Buffer (5X), 1x500ul. 250mM Tris-HCL, pH 8.0, 50% glycerol, 0.5mM EDTA and 5mM DTT.\nH1923-01B: Core Histones, 1x200ug. Chicken erythrocyte core histones lyophilized from sterile distilled water. Aseptically reconstitute to 1mg/ml in sterile water and aliquot to avoid repeated freezing and thawing.\nH1823-01C: P81 Posphocellulose squares. 100 prelabeled cellulose phosphate paper squares. Strong cation exchanger of high\ncapacity; ion exchange capacity is 18.0 mEq/cm2. Thin (0.17 mm) paper. Flow rate: 125 mm/30 min. Very useful for the separation of biogenic amines, antibiotics, histamines, certain metals (Fe, Cu) at very low concentrations.\nH1823-01D: Acetyl-C0A, 10x10ug. Each vial contains 10ug of Acetyl-coenzyme A, trilithium salt, lyophilized from 25ul sterile distilled water. Acetyl-CoA is a carrier of acetyl groups for enzymatic reactions involving biological acetylation and fatty acid synthesis and oxidation.\n\nMaterials Required But Not Provided:\n[3H] Acetyl-CoA (Amersham, Catalog # TRK688, 290 Ci/mmol) \nHistone acetyltransferase \nTrichloroacetic acid \nEthanol \nSterile distilled water \nScintillation fluid \nMicrocentrifuge tubes \nMicropipettes and tips \nMicrocentrifuge \nTimer \nScintillation vials \nScintillation counter \nRotating wheel/platform \n\nStorage and Stability: \nH1823-01A: Store at -20 degrees C.\nH1923-01B: Store at -20 degrees C. Folowing reconstitution, aqueous solutions are stable for 6 months at -70 degrees C.\nH1823-01C: Store at -20 degrees C\nH1823-01D: Store at -20 degrees C. Following reconstitution, aqueous solutions are stable for 1-2 weeks at -20 degrees C. Discard and reconstitute fresh vial after 2 weeks.\n

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