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HAT1, aa20-341, Recombinant, Human (Histone Acetyltransferase 1, Histone Acetyltransferase Type B Catalytic Subunit, KAT1)

Cat no: H1823-05

HAT1, aa20-341, Recombinant, Human (Histone Acetyltransferase 1, Histone Acetyltransferase Type B Catalytic Subunit, KAT1)

HAT1, also known as histone acetyltransferase 1, is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Recombinant HAT1 protein was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa20-341 of human HAT1 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MKKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1823-05

Size

50ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.

Purity

~ 90% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Verreault A., et al. (1998) Curr Biol. 8(2):96-108. 2. Marmorstein R., et al. (2001) J Mol Biol. 311(3):433-44.

Alternative Names

EC=2.3.1.48

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