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HBEGF BioAssay(TM) ELISA Kit, Human

Cat no: 143860

HBEGF BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human HBEGF in cell culture supernates, serum and plasma (heparin, EDTA).\n\nHBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 10 pg/ml \n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ99075\n\nEntrez Gene ID:\n1839\n\nKit Components:\n96-well plate pre-coated with anti-HBEGF, Human\nLyophilized recombinant HBEGF, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143860

Size

96Tests

References

1. Nanba D, Higashiyama S (February 2004). \"Dual intracellular signaling by proteolytic cleavage of membrane-anchored heparin-binding EGF-like growth factor\". Cytokine Growth Factor Rev. 15 (1): 13

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