Hck, phosphorylated (Tyr209, Ser211) (Hematopoietic Cellular Kinase)

Hck is a member of the Src family of non-receptor tyrosine kinases involved in the modulation of a wide variety of normal and pathogenic cellular processes including growth, development, differentiation and carcinogenesis. These proteins contain a consensus structure comprised of one or more N-terminal SH2 and/or SH3 domains involved in protein-protein interactions, a central catalytic tyrosine kinase domain and a short C-terminal domain. This C-terminal domain contains a tyrosine residue (tyrosine 522) that, when phosphorylated by Csk tyrosine kinase, functions as a negative regulatory site by providing a binding site for the N-terminal SH2 domain. The association of these two domains blocks access to a critical tyrosine residue in the activation loop, the phosphorylation of which is required for enzyme activation. Release of this autoinhibition can be achieved either by dephosphorylation of tyrosine 522 or by phosphorylation of tyrosine 209 and/or serine 211 in the SH2 domain. \n\nApplications: \nSuitable for use in ELISA and Western Blotting. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls: \nPC12 cells +/- sorbitol, Chick Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin and recombinant activated Fyn, Lck and Lyn proteins.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1827-10

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Young, M.A., et al. (2001) Dynamic coupling between the SH2 and SH3 domains of c-Src and Hck underlies their inactivation by C-terminal tyrosine phosphorylation. Cell 105(1):115-126. 2. Hubbard, S.R. and J.H. Till (2000) Protein tyrosine kinase structure and function. Annu. Rev. Biochem. 69:373-398. 3. Thomas, S.M. and J.S. Brugge (1997) Cellular functions regulated by Src family kinases. Annu. Rev. Cell Devel. Biol. 13:513-609. 4. Stover, D.R., et al. (1996) Modulation of the SH2 binding specificity and kinase activity of Src by tyrosine phosphorylation within its SH2 domain. J. Biol. Chem. 271(21):12481-12487.

Additional Info

Recognizes Rat Hck when phosphorylated at tyrosine 207 and serine 209. Species sequence homology: human (92%) and mouse (100%) There is some cross-reactivity with Src family kinases Lck and Lyn, but the antibody does not react with Src, Fyn, or Yes.