HCV, NS3 (Nonstructural Protein 3)

The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.\n\nApplications:\nSuitable for use in Immunocytochemistry, Western Blot\n\nRecommended Dilution:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

044880

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

>95% (SDS-PAGE)

Isotype

IgG1

References

Product Reference: \n?Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines: B. Wolk, et al.; J. Virol. 74, 2293 (2000)\n?Protein-protein interactions between hepatitis C virus nonstructural proteins: Dimitrova, et al.; J. Virol. 77, 5401 (2003)\n?Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons: R. Gosert, et al.; J. Virol. 77, 5487 (2003)\n?Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes: D. Moradpour, et al.; J. Virol. 78, 7400 (2004)\n?Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005)\n?Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005)\n?

Additional Info

Recognizes HCV NS3 (genotype 1a and 1b) (epitope mapped to NS3 aa 160-193), recognizes NS3 alone or the NS3-4A complex. Does not cross-react with HCV NS3 (genotype 2a). t

Alternative Names

Nonstructural Protein 3