HCV, NS5B (blocking) (Nonstructural Protein 5B)

The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.\n\nApplications:\nSuitable for use in Neutralization, Immunocytochemistry, Immunoprecipitation\n\nRecommended Dilution:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

044881

Size

100ug

Applications

ICC, IP

Hosts

Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Mab

Purity

>95% (SDS-PAGE)

Isotype

IgG2a

References

Product Reference: \n?Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase: J. Schmidt-Mende, et al.; J. Biol. Chem. 276, 44052 (2001)\n?Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002)\n?Protein-protein interactions between hepatitis C virus nonstructural proteins: Dimitrova, et al.; J. Virol. 77, 5401 (2003)\n?Interference of hepatitis C virus RNA replication by short interfering RNAs: Kapadia, et al.; PNAS 100, 2014 (2003)

Additional Info

Recognizes HCV NS5B from HCV genotype 1 (epitope mapped to NS5B aa 139-392). Does not react with genotype 2.

Alternative Names

Nonstructural Protein 5B