HDAC, Mammalian, BioAssay(TM) Activity Assay Kit (Fluorometric)

Supplier:

United States Biological

Cat no: 167855

HDAC, Mammalian, BioAssay(TM) Activity Assay Kit (Fluorometric)

Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription and induce apoptosis or differentiation in cancer cells. However, screening compounds for HDAC inhibition has been difficult due to the lack of convenient tools for analyzing HDAC activity. The Fluorometric HDAC Activity Assay Kit provides a fast and fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays. The new procedure requires only two easy steps, both performed on the same microtiter plate. First, the HDAC substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract). Deacetylation of the substrate sensitizes the substrate, so that further treatment with the Lysine Developer produces a fluorophore. The fluorophore can be easily analyzed using a fluorescence plate reader or a fluorometer. The assay is well suited for high throughput screening applications. HDAC inhibitors and antibodies are also available separately.\n\nSample Type:\nCell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids.\n\nIntended Use:\nMeasurment of HDAC in mammal tissue and cell samples:\n\nSpecificity:\nRecognizes mammalian HDAC\n\nKit Components:\nHDAC substrate (4mM), 1x0.5ml\n10X HDAC assay buffer, 1x1ml\nHDAC inhibitor (1mM), 1x10ul\nHeLa Nuclear Extract (5mg/ml), 1x10ul\nDeacetylated standard (4mM), 1x20ul\n\nStorage and Stability:\nStore liquid at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167855

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

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