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HDAC1, Active, Recombinant, P. Falciparum, His-FLAG-Tag (Histone Deacetylase 1, RPD3L1)

Cat no: 137276

HDAC1, Active, Recombinant, P. Falciparum, His-FLAG-Tag (Histone Deacetylase 1, RPD3L1)

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.\n\nSource:\nRecombinant corresponding to aa1-450 from Plasmodium falciparum HDAC, fused to Flag-His-tag at C-terminal, expressed in a Baculovirus infected Sf9 cell expression system.\n\nMolecular Weight:\n~53.4kD\n\nApplications:\nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nSpecific Activity:\n~1.66pmol/min/ug\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137276

Size

20ug

Form

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 90ug/ml FLAG peptide, 10% glycerol.

Purity

Purified (~58%)

References

1. Chen et al., J. Med. Chem. 2008 Jun 26; 51(12):3437-48. 2. Joshi et al., Mol. Biochem. Parasitol. 1999 Mar 15; 99(1):11-9.

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