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HDAC1, Recombinant, Human, His-FLAG (Histone Deacetylase 1)

Cat no: H1827-50E

HDAC1, Recombinant, Human, His-FLAG (Histone Deacetylase 1)

Description:\nHuman HDAC1 (GenBank Accession No. NM_004964), full length with C-terminal His-FLAG tag.\n\nSpecific Activity: \n~400pmol/min/ug\n\nAssay Conditions: \n25mM Tris/Cl, pH 8.0, 137mM sodium chloride,2.7mM KCl, 1mM MgCl2, 0.1mg/ml BSA, 20uM BPS HDAC substrate, HDAC1 \n\nIncubation conditions:\n30 minutes at 37C followed by treatment with 50ul of HDAC developer, Catalog #H5109-66B for 15 minutes at RT\n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications have not been tested. \n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

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SPECIFICATIONS

Catalog Number

H1827-50E

Size

50ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 80ng/ul FLAG peptide, 20% glycerol

Purity

(same/more than)50% by SDS-PAGE

References

1. Rikimaru,T. et al., Oncology 72 (1-2), 69-74 (2007). 2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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