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HDAC10, Active, Recombinant, Human, FLAG-Tag (Histone Deacetylase 10, HD10)

Cat no: 137277

HDAC10, Active, Recombinant, Human, FLAG-Tag (Histone Deacetylase 10, HD10)

HDAC10 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.\n\nSource:\nRecombinant corresponding to aa2-631 from human HDAC10, fused to Flag-tag at N-terminal, expressed in Sf9 cells via a baculovirus expression system.\n\nMolecular Weight:\n~67kD\n\nApplications:\nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nSpecific Activity:\n~3.6pmol/min/ug\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137277

Size

50ug

Form

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 0.05% Tween-20, 10% glycerol.

Purity

Purified (~50%)

References

1. Chakraborty S. (2006) J. Biol. Chem. 281(46):35070-80. 2. Wolfgang, F. (2001) J. Biol. Chem. 276(38): 35826-35835.

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