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HDAC2, Recombinant, Human, aa1-488, His-tag (Histone Deacetylase 2, Tanscriptional Regulator Homolog RPD3, HD2)

Cat no: H1827-53E

HDAC2, Recombinant, Human, aa1-488, His-tag (Histone Deacetylase 2, Tanscriptional Regulator Homolog RPD3, HD2)

HDAC2 belongs to the histone deacetylase family that act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones. It forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. It also plays an important role in transcriptional regulation, cell cycle progression and developmental events. Purified by using conventional chromatography techniques.\n\nSource:\nRecombinant corresponding to aa1-488 of human HDAC2, expressed with c-terminal His-tag in high-5 cells using baculovirus expression system.\n\nAA Sequence:\nMAYSQGGGKK KVCYYYDGDI GNYYYGQGHP MKPHRIRMTH NLLLNYGLYR KMEIYRPHKA TAEEMTKYHS DEYIKFLRSI RPDNMSEYSK QMQRFNVGED CPVFDGLFEF CQLSTGGSVA GAVKLNRQQT DMAVNWAGGL HHAKKSEASG FCYVNDIVLA ILELLKYHQR VLYIDIDIHH\nGDGVEEAFYT TDRVMTVSFH KYGEYFPGTG DLRDIGAGKG KYYAVNFPMR DGIDDESYGQ IFKPIISKVM EMYQPSAVVL QCGADSLSGD RLGCFNLTVK GHAKCVEVVK TFNLPLLMLG GGGYTIRNVA RCWTYETAVA LDCEIPNELP YNDYFEYFGP DFKLHISPSN MTNQNTPEYM\nEKIKQRLFEN LRMLPHAPGV QMQAIPEDAV HEDSGDEDGE DPDKRISIRA SDKRIACDEE FSDSEDEGEG GRRNVADHKK GAKKARIEED KKETEDKKTD VKEEDKSKDN SGEKTDTKGT KSEQLSNPSR HHHHHH\n\nMolecular Weight: \n~56.4kD (496aa)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1827-53E

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 0.1M sodium chloride, 1mM DTT, 0.1mM PMSF, 20% glycerol.

Purity

~85% (SDS-PAGE)

References

Betz R. et al. (1998) Genomics. 52:245-6. Taunton J. et al. (1996) Science 272:408-11.

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