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HDAC2, Recombinant, Human (Histone Deacetylase 2, Tanscriptional Regulator Homolog RPD3, HD2)

Cat no: H1827-53B

HDAC2, Recombinant, Human (Histone Deacetylase 2, Tanscriptional Regulator Homolog RPD3, HD2)

Human, recombinant, full length with C-terminal His tag expressed in Sf9 cells.\n\nApplications:\nSuitable for use in enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.\n\nSpecific Activity:\n~400pmol/min/ug\n\nRecommended Dilution:\nAssay Conditions: \n50mM Tris-HCl, pH 8.0, 137mM sodium chloride, 2.7mM of KCl, 1mM MgCl2, 20uM substrate, 5ug BSA, H1827-53B. Incubate at 37C for 30 minutes.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

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SPECIFICATIONS

Catalog Number

H1827-53B

Size

50ug

Form

Supplied as a liquid in 40mM Tris, pH 8.0, 100mM sodium chloride, 2.2mM KCl, 0.04% Tween-20, 20% glycerol.

Purity

~ 70% by SDS-PAGE

References

1. M.H. Kuo & C.D. Allis (1998) Bioessays 20:615. 2. Y. Zeng, et al. (1998) J. Biol. Chem. 273:28921. 3. S. Sasaki, et al. (1999) EMBO J. 18:5389.

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