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HDAC4 (Histone Deacetylase 4, HA6116, HD4, HDACA, Histone Deacetylase A, KIAA0288)

Cat no: H1827-55K3

HDAC4 (Histone Deacetylase 4, HA6116, HD4, HDACA, Histone Deacetylase A, KIAA0288)

Cellular Localization: Cytoplasmic and Nuclear\n\nPositive Control: Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cells (Western blotting) and HeLa nucle\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1827-55K3

Size

50ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

15mM Sodium Azide; 1% BSA, 0.01M PBS. pH 7.4

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). <PUBMED:11467747>Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). <PUBMED:11250139>Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). <PUBMED:10523670>Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999). <PUBMED:10220385>

Additional Info

Species Crossreactivity: Crossreacts with Human, Mouse and Rat. Expected to crossreact with Chicken (93% identity with immunogen) due to sequence homology. Not yet tested in other species.

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