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Heat Shock Protein 27, CT (HSP27)

Cat no: H1830-50A

Heat Shock Protein 27, CT (HSP27)

Heat shock protein (HSP) 27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells. It belongs to the small heat shock protein (HSP 20) family. Within minutes of heat shock, HSP27 is phosphorylated, and relocalizes from the cytoplasm to within or around the nucleus[1,2]. \n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:500 detected HSP27 in 20ug of L6 cell lysates untreated and treated with anisomycin (10ug for 30 min). \nImmunocytochemistry: 10ug/ml detected nuclear staining in heat shocked CHO cells fixed in 50% ethanol/50% acetic acid.\nOptimal dilution determined by the researcher. \n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\nI1904-40D: IgG, H&L (FITC) Pab Gt xRb\nI1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb \nI1904-46N: IgG, H&L (FITC) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-50A

Size

250ug

Applications

ICC, WB

Hosts

Rabbit

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Arrigo, A.P. and J. Landry (1994) In R.I. Morimoto, et al. (ed.), The biology of heat shock proteins and molecular chaperons, p.335

Additional Info

Recognizes rodent HSP 27 (27kD). No crossreactivity was observed with human HSP 27.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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