Heat Shock Protein 70 (HSP70)

Heat shock proteins are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. Eukaryotic cells contain an HSP70 multigene that differ in their intracellular location and regulation. Members of the HSP70 family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes, as well as in the immune response. HSP72 was found to increase dramatically in the brains of Alzheimer?s disease patients, and was localized exclusively in neuritic plaques and neurofibrillary tangles. HSP70 concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: Immunofluorescent staining demonstrates a rapid and reversible accumulation of HSP70 within the nucleus of heat-stressed (42C, 1 hour) human fibroblasts. \nWestern Blot: 1:5000\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-94G

Size

100ul

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Bov, Ch/Bird, Dro/Arth, GP, Rabbit

Form

Supplied as a liquid, 15mM sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Wimmer B et al. Proc Natl Acad Sci USA 94:13624-9 (1997). 2. Velez-Granell CS et al. J Cell Sci 107 (Pt 3):539-49 (1994). 3. Marchesi VT & Ngo N. Proc Natl Acad Sci USA 90:3028-32 (1993). 4. Hamos JE et al. Neurology 41:345-50 (1991). 5. Kaufmann SH. Immunol Today 11:129-36 (1990). 6. Omar R et al. Arch Pathol Lab Med 114:589-92 (1990). 7. Mizzen LA et al. J Biol Chem 264:20664-75 (1989). 8. Reading DS et al. Nature 337:655-9 (1989). 9. Reading DS et al. Nature 337:655-9 (1989). 10. Lindquist S & Craig EA. Annu Rev Genet 22:631-77 (1988). 11. Pelham HR. Cell 46:959-61 (1986). 12. Velazquez JM & Lindquist S. Cell 36:655-62 (1984).

Additional Info

Recognizes bovine Heat Shock Protein 70 (HSP70) from brain and other tissues. In Western Blot, localizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species Crossreactivity: rat, chicken, human, Drosophila melanogaster, guinea pig, hamster, plants, rabbit, C. elegans.