Heat Shock Protein 70 (HSP70)

Hsp70B (heat shock protein 70B) is a nuclear-encoded, chloroplast-targeted chaperone of the Hsp70 family. It is the major Hsp70 in the stroma of Chlamydomonas chloroplasts. It interacts with Hsp90C, CGE1, CDJ2 and VIPP1 (1-3). It has been shown that using Chlamydomonas \nreinhardtii as plant model organism the alga encodes a HEP homolog (termed HEP2) that is localized to the stroma (4). HEP2 is expressed constitutively as a low abundance protein with an apparent molecular mass of ~21kD. In cell extracts HEP2 interacts with HSP70B in an ATP-dependent fashion. Coexpression of HSP70B with HEP2 in E. coli yields high levels of CGE1-binding competent HSP70B, which also displayed ATPase activity (4). Inactive HSP70B was more prone to proteolysis than active HSP70B. Although inactive HSP70B interacts with HEP2, it is not activated. Active HSP70B remains active for 48 h in the absence of HEP2, \nsuggesting that HEP2 was not involved in maintaining HSP70B in an active state. However, it was found that some HSP70B expressed as a fusion protein with an N-terminal extension \nwas activated when HEP2 was present during cleavage of the fusion protein, suggesting that in vivo HEP2 might be required for renewed folding of HSP70B after transit peptide cleavage \n(4). \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1:10000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1830-95T6

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Form

Supplied as a lyophilized powder. Reconstitute with 100ul sterile water.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Schroda M., Vallon O., Wollman F.A. and Beck C.F. (1999) Plant Cell. 11:1165-1178. 2. Schroda M et al. (2001) Plant Cell. 13:2823-2839. 3. Liu C., et al. (2005) Mol Biol Cell. 16:1165-1177. 4. Willund, F. et al. (2008) J. Biol. Chem., Vol. 283, Issue 24,16363-16373.

Additional Info

Recognizes Hsp70B. Species Crossreactivity: Chlamydomonas reinhardtii.