Heat Shock Protein 70, Mitochondrial (HSPA9B, mtHSP70, GRP75, Mortalin 2)

The 70kD heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and \nconstitutively expressed members known as heat shock As reportedcognate proteins. Glucose Regulated 75kD Protein (is a 679 amino acid heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. GRP75 is a mitochondrial protein involved in protein translocation into the mitochondria. Proteins crossing the mitochondrial membrane require unfolding before entering translocation pores in the mitochondrial outer membrane. GRP75 together with other inner membrane proteins of the mitochondria mediate this process. GRP75 also plays a role in the control of cell cycle progression. \n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 0.02% SODIUM AZIDE. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1830-91B

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from 20mM Tris, pH 7.4, 0.15M sodium chloride, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% SODIUM AZIDE.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG3

Additional Info

Recognizes endogenous human GRP75. Species Crossreactivity: mouse and rat. Does not crossreact with recombinant human HSPA1A, HSPA2, HSPA6, HSPA8 or GRP78 in Western Blot.