Heat Shock Protein 90 (HSP90)

In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml. Detects a band at ~90kD.\nImmunohistochemistry (frozen and paraffin): 1:10-1:2000\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: \nCytoplasmic\n\nPositive Control: \nHeLa Cell Lysate (Heat Shocked). Hsp90 Protein.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1831-58B

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Feng H et al. Blood 100:4108-15 (2002). <PUBMED:12393401> 2. Gebhard B et al. Anticancer Res 19:5293-7 (1999). <PUBMED:10697551>Imamura T et al. Involvement of heat shock protein 90 in the degradation of mutant insulin receptors by the proteasome. J Biol Chem 273:11183-8 (1998). <PUBMED:9556607> 3. Smith DF et al. Mol Cell Biol 13:869-76 (1993). <PUBMED:8423808> 4. Redmond T et al. Eur J Cell Biol 50:66-75 (1989). <PUBMED:2693091> 5. Riehl RM et al. Biochemistry 24:6586-91 (1985). <PUBMED:4084541>

Additional Info

Recognizes both Hsp90 alpha and beta. It reacts with the rodent glucocorticoid receptor and with proliferation potential proteins (P2PS) with apparent molecular masses of 30-40kD, which are associated with the 30-40S substructures of nuclear hnRNP complexes and share an epitope in common with Hsp90. Species Crossreactivity: human, rat, mouse, bacteria, beluga, canine, chicken, c. elegans, fish, guinea pig, hamster, monkey, mussel, porcine, rabbit, sheep, scallop and water mold HSP90.