Hepatitis B surface antigen (HBsAg) is a serological marker on the surface of hepatitis B virus. It is a group of morphologically heterogeneous complex macromolecular structures found in the serum of patients with hepatitis B virus infection. Electron microscopy reveals three forms of HBsAg, 20nm spherical particles, tubular structures of variable lengths and a diameter of 20nm, and 40nm spherical "Dane" particles which are the hepatitis B virus. Purified HBsAg has a density of 1.20-1.21 g/ml. HBsAg usually contain protein, carbohydrate, and lipid. HBsAg particles carry the common determinant, a, as well as d or y and w or r subtype determinants, and are classified into the four major subtypes, adw, adr, ayw and ayr. Rare sera contain HBsAg particles with all four subtype determinants (adywr).
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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