Hepatitis C Virus NS5, Recombinant (HCV) (HRP)

The hepatitis C virus (HCV) NS5A gene product is a phosphorylated 56- to 58kD nonstructural protein that displays a multitude of activities related to enhancement of viral pathogenesis. Although associated with other viral encoded proteins as part of the viral replicase complex positioned on the cytoplasmic side of the endoplasmic reticulum, a role for NS5A in viral replication has not been defined. Truncated versions of NS5A can act as transcriptional activators, while other recently characterized interactions of NS5A with cellular proteins indicate its pleiotropic role in HCV-host interactions.\n\nIt has been shown that NS5B is a membrane-associated protein, which contains a C-terminal domain comprising R21 hydrophobic amino acids that is responsible for membrane anchorage. NS5B may form a complex with cellular proteins or other HCV nonstructural proteins, including NS3, the viral protease and helicase; NS4A, a cofactor of NS3 protease activity; and NS5A, a phosphoprotein containing a putative interferon sensitivity region. Although the HCV replication mechanism is not clearly understood, the essential role of NS5B polymerase in the HCV replication and infection process has been demonstrated in chimpanzees. Accordingly, it has been viewed as an attractive target for antiviral intervention.\n\nSpecificity:\nImmunoreactive with sera of HCV-infected individuals.\n\nApplications:\nRecombinant HCV NS5 Antigen may be used in ELISA and Western Blot, excellent for detection of HCV with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1920-27C2

Size

100ug

Form

Supplied as a liquid in 25mM Tris-HCl, 1mM EDTA, 1.5M urea, 50% glycerol. Conjugated to HRP.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. HCV NS5b RNA-dependent RNA polymerase inhibitors: from alpha,gamma-diketoacids to 4,5-dihydroxypyrimidine- or 3-methyl-5-hydroxypyrimidinonecarboxylic acids. Design and synthesis. Summa V, Petrocchi A, Taliani M, Pace P, J Med Chem 2004 Oct 21;47(22):5336-9 2. HCV NS5A abrogates p53 protein function by interfering with p53-DNA binding. Gong GZ, Jiang YF, Lai LY, World J Gastroenterol 2004 Aug 1;10(15):2223-7 3. Evaluation of a prototype HCV NS5b assay for typing strains of hepatitis C virus isolated from Tunisian haemodialysis patients. Othman SB, Trabelsi A, Bouzgarrou N, Pozzetto B, J Virol Methods 2004 Aug;119(2):177-81 4. The monoethyl ester of meconic acid is an active site inhibitor of HCV NS5B RNA-dependent RNA polymerase. Pace P, Nizi E, Pesci S, Summa V, Bioorg Med Chem Lett 2004 Jun 21;14(12):3257-61 5. Cellular RNA helicase p68 relocalization and interaction with the hepatitis C virus (HCV) NS5B protein and the potential role of p68 in HCV RNA replication. Goh PY, Tan YJ, Tan YH, J Virol 2004 May;78(10):5288-98 6. Discovery of thiophene-2-carboxylic acids as potent inhibitors of HCV NS5B polymerase and HCV subgenomic RNA replication. Part 2: tertiary amides. Chan L, Pereira O, Das SK, Siddiqui A, L'Heureux L, Bioorg Med Chem Lett 2004 Feb 9;14(3):797-800.