Glycosidic bonds rarely affect the function of glycoproteins, thus they are usually not necessary to determine. However, the glycan structure of glycoproteins is highly complex, and assessing the configuration and location of N-glycan glycosidic bonds is helpful for analyzing the structure of glycoproteins. There are five common types of N-glycosidic bonds, with the most common being the connection between N-acetylglucosamine (GlcNAc?1-Asn) and asparagine (Asn). Other types connected to Asn include glucose
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