Histamine, BioAssay(TM) ELISA Kit

In humans, histamine (b-imidazolethylamine) is the most important mediator and is mostly found in the initial phase of an anaphylactic reaction ("immediate type" allergy). Histamine is derived by the enzymatic decarboxylation of histidine. In the organism, histamine is present in nearly all tissues, and it is mainly stored in the metachromatic granula of mast cells and the basophilic leukocytes. It is present in an inactive bound form and is only released as required. Like several other mediators, histamine does not only mediate various clinical symptoms of anaphylaxis but also induces a series of effects which are directed towards a termination of the anaphylactic reaction. The biological action of histamine in tissue is guaranteed by three\ndifferent surface receptors, i.e. H1, H2 and H3 receptors. Of clinical interest in the histamine determination is the quantification of the histamine release from basophilic leukocytes in allergies of the "immediate type" as well as of the histamine quantity which is present in various body fluids (plasma, urine, cell culture supernatants), after allergen administration.\n\nSample Type:\nPlasma, Urine, Cell Structure\n\nIntended Use:\nEnzyme immunoassay for the quantitative determination of histamine in human plasma and urine. Further the test can be used for research of cell culture supernatants.\n\nTest Principle:\nSolid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.\n\nKit Components:\nMicrotiter Plate (MTP) Coated with anti-rabbit antiserum (goat), 1x96 wells\nHistamine Antiserum 1x5ml \nEnzyme Conjugate Concentrate (200X), 1x75ul\nPlasma Standard, 7x400ul\nPlasma Control 1, 1x1vial\nPlasma Control 2, 1x1vial\nUrine/Cell Culture Standard, 0ng/ml, 1x250ul\nUrine/Cell Culture Standard, 2.7ng/ml, 1x250ul\nUrine/Cell Culture Standard, 8.1ng/ml, 1x250ul\nUrine/Cell Culture Standard, 24.3ng/ml, 1x250ul\nUrine/Cell Culture Standard, 73ng/ml, 1x250ul\nUrine/Cell Culture Standard, 219ng/ml, 1x250ul\nUrine Control 1, 1x250ul\nUrine Control2, 1x250ul\nAcylation Reagent, 1x1.5ml\nAssay Buffer Concentrate (5x), 1x60ml\nWash Buffer Concentrate (20x), 1x50ml\nIndicator Buffer, 1x10ml\nTMB Substrate Solution Concentrate (31x), 1x0.9ml\nTMB Substrate Buffer, 1x27ml\nTMB Stop Solution, 1x12ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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