Histone H1, phosphorylated

Histones are a family of basic proteins found in all eukaryotic nuclei that are associated with eukaryotic DNA. The five major types of histone are H1, H2A, H2B, H3 and H4. The latter four, which comprise nucleosome core, are evolutionarily conserved, while the linker histone H1 exhibits greater sequence diversity among organisms. The amino acid sequence of H1 varies more from organism to organism. The separation of H1 into multiple bands by gel electrophoresis results from differences in the extent of phosphorylation of residues in the protein.\n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml detected phosphorylated Histone H1 from acid extracts of colcemid treated HeLa cells. Does not detect Histone H1 in untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-03 (2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit. A chemiluminescence detection system was used. \nImmunocytochemistry: 5-10ug/ml showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol, 5% glacial acetic acid.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H5110-03

Size

200ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Dedon, P.C., et al., Anal. Biol. Chem. 197: 83-90, 1991. 2. Pennisi, E., Science 275: 155-157, 1997. 3. Morita, E., et al., J. Virol. 15: 7555-7563, 2001. 4. Bhattacharjee, R., et al., Mol. Cell. Biol. 21: 5417-5425, 2001. 5. Lu, M.J., et al., Chromosoma 103: 111-121, 1994.

Additional Info

Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species Crossreactivity: human