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Histone H2B ELISA Kit

Cat no: ABIN1874274


Supplier: Antibodies-online
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Histone H2B ELISA Kit: The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm
Catalogue number: ABIN1874274
Size: 96 tests
Gene: 680312
Antigen: Histone H2B (H2B)
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 3.125-200 ng/mL
Sample type: Tissue Homogenate,Cell Lysate,Biological Fluids
Entrez gene: 680312

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