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Histone H4 ELISA Kit

Histone H4 ELISA Kit

Cat no: ABIN1874266

Supplier: Antibodies-online
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Histone H4 ELISA Kit: The microtiter plate provided in this kit has been pre-coated with an antibody specific to H4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm
Catalogue number: ABIN1874266
Size: 96 tests
Gene: 8370,97122
Antigen: Histone H4 (H4)
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 0.156-10 ng/mL
Sample type: Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids
Entrez gene: 8370,97122

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