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HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)

Cat no: H6007-10B

HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)

Coupling Ratio: \n~10-20 moles of biotin per mole tat. \n\nBiological Activity: \nBiotin tat binds to solid phase murine anti-tat mAb as determined by capture ELISA using strepavidin-HRP as a secondary reagent.\n\nApplications:\nA dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1:1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. tat/anti-tat mAb capture ELISA may be performed in 100ng mAb in solid phase and 1-10ng of biotin-tat.\n\nStorage and Stability:\nStable for 6 months at 4 degrees C.

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SPECIFICATIONS

Catalog Number

H6007-10B

Size

50ug

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.01% Thimersol. Conjugated to Biotin.

Purity

Purified by ion affinity and reverse phase HPLC to (same/more than) 99% as determined by SDS-PAGE and HPLC.

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