HLA Class 1 Antigen A11

Applications:\nThis antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.\n\nRecommended Dilutions:\nCytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution. \nOptimal dilutions to be determined by the researcher.\n\nCytotoxicity:\nExpected Results:\nCell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.\nLimitations:\nCell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.\n\nSource:\nAscites fluid\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H6098-28

Size

100ul

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

1. NCCLS Tentative Standard. \"Leukocyte Differential Counting\" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200

Additional Info

Recognizes human HLA Class 1 Antigen-A11. \nCytotoxicity Crossreactivity: HLA class 1, A3. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.