hnRNP A1 (Heterogeneous Nuclear Ribonucleoprotein A1, Helix Destabilizing Protein, Heterogeneous Nuclear Ribonucleoprotein Core Protein A1, hnRNP Core Protein A1, HNRPA1, MGC102835, Nuclear Ribonucleoprotein Particle A1 Protein, Single Strand DNA Binding

RNA polymerase II trans cripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, premRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts.2, 4, 5 Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins\n\nof the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34kD up to 43kD.2, 4, 5 hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein binds to its RNA target through a consensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP -A1 from transportin. hnRNP-A1 is ubiquitously expressed with a higher expression in proliferating and/or transformed cells than in differentiated tissues.6\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H6282-01

Size

50ul

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Bov, Can

Form

0.01 M PBS, pH 7.4

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2a

References

1. Pinol-Roma, S., et al., Genes Dev., 2, 215-227 (1988).\n2. Burd, C.G., et al., EMBO J., 13, 1197-1204 (1994).\n3. Vautier, D., et al., J. Cell Sci., 114, 1521-1531 (2001).\n4. Siomi, M.C., et al., J. Cell Biol., 138, 1181-1192 (1997).\n5. Izaurralde, E., et al., J. Cell Biol., 137, 27-35 (1997).\n6. Iervolino, A., et al., Mol. Cell. Biol., 22, 2255- 2266 (2002).

Additional Info

Detects a band at approximately 32-35kD. This antibody immunoprecipitates the hnRNP complex. Species Crossreactivity: Crossreacts with Human, bovine and canine.