hnRNP C1, C2 (Heterogeneous Nuclear Ribonucleoprotein C1/C2, hnRNP C1 / hnRNP C2, hnRNPC, MGC104306, MGC105117, MGC117353, MGC131677, Nuclear Ribonucleoprotein Particle C1 Protein, Nuclear Ribonucleoprotein Particle C2 Protein, SNRPC)

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34kD up to 43kD. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H6282-50

Size

50ul

Applications

ELISA, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Ch/Bird, NHP

Form

0.01 M PBS [pH 7.4] containing 15mM sodium azide

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Choi, Y.D., and Dreyfuss, G., Proc. Natl. Acad. Sci. USA, 81, 7471-7475 (1984).\n2. Pinol-Roma., S., et al., J. Cell Biol., 109, 2575-2587 (1989).\n3. Krecic, A.M., et al., Curr. Opin. Cell Biol., 11, 2363-2371 (1999).\n4. Williamson, D.J., et al., Mol. Cell. Biol., 20, 4094-4105 (2000).

Additional Info

This antibody detects a band at approximately 41 and 43kDa. Species Crossreactivity: Crossreacts with Human, Chicken, Hamster and Monkey.