hnRNP H (HNRNPH1, hnRNPH1, Heterogeneous Nuclear Ribonucleoprotein H)

hnRNP H1 is a member of the hnRNP family, which associate with heterogeneous nuclear RNA in the nucleus and are involved in the regulation of pre-mRNA processing. Alternative pre-mRNA splicing is frequently used to expand the protein-coding capacity of genomes, and to regulate gene expression at the post-transcriptional level. The inherent flexibility of these mechanisms is specified by numerous short sequence motifs distributed in introns and exons. Whereas hnRNP (heterogeneous nuclear ribonucleoprotein) A1 mediates silencing of the CI cassette exon in conjunction with the guanosine-rich motifs, hnRNP H functions as an antagonist to silencing. Genome-wide analysis shows that, while this motif pattern is rarely present in human and mouse exons, those exons for which the pattern is conserved are generally found to be skipped exons. The identification of a similar arrangement of guanosine-rich motifs in transcripts of the hnRNP H family of splicing factors has implications for their co-ordinate regulation at the level of splicing.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1: 5.000-1: 15,000\nImmunoprecipitation: 4-10 ug/mg protein\nOptimal dilution determined by the researcher. \n\nRecommended Secondary Reagents: \nI1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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