hnRNP K (Heterogeneous Nuclear Ribonucleoprotein K, CSBP, dC Stretch Binding Protein, FLJ41122, hnRPK, Transformation Up-regulated Nuclear Protein, TUNP)

The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants 1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene \nexpression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3). \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100,000 \nImmunoprecipitation: 1:50 \nImmunohistochemistry: 1:100-1:250 \nImmunocytochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

H6285-31A

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

1. Dejgaard K, et al. J Mol Biol. 236(1):33-48, 1994. 2. Mikula M, et al. Proteomics (8):2395-406, 2006. 3. Carpenter B, et al. Br J Cancer 95(7):921-7, 2006

Additional Info

Recognizes human both phosphorylated and unphosphorylated hnRNP K. Species Crossreactivity: Mouse and rat.