HNRNPM (Heterogeneous Nuclear Ribonucleoprotein M, hnRNP M, HNRPM, NAGR1)

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.\n\nApplications:\nSuitable for use in Western Blot and RNA Binding Protein Immunoprecipitation assay. Other applications not tested.\n\nRecommended Dilution:\nRIP: ~5ug of antibody per RIP \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nIncludes negative control normal mouse IgG antibody and control primers specific for the cDNA of human hnRNP M.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029506

Size

10Tests

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Datar, K.V., et al. (1993). Nucleic Acids Research 21:439-446. 2. Kiesler, E., et al. (2005). J. Cell Biol. 168:1013-1025. 3. van Wezel, T., et al. (2005). Breast Cancer Res. 7:R998-R1004.

Additional Info

Recognizes human HNRNPM. Species sequence homology: Bovine, mouse, porcine, rat, rabbit.