HOP, p60, Recombinant, Human (HSP70 HSP90 Organizing Protein)

Hsp70-Hsp90 Organizing Protein (HOP, p60) is an ~60kD protein that is a critical intermediate component for the efficient maturation of steroid receptor complexes (1-3), serving to recruit Hsp90 to Hsp70-containing complexes. Unactivated steroid hormone receptors are found in hetero-oligomeric complexes that are thought to stabilize a partially folded receptor polypeptide prior to hormone-dependent activation (4-6). Hsp70 and Hsp90 are both known to associate with steroid hormone receptors. Numerous studies (8-13) have shown that Hsp70 functions in an ATP-dependent manner through transient interactions to mediate folding or unfolding of polypeptide chains. Hsp90 is thought to perhaps also function in some capacity related to folding or protein-protein interactions (14). HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. Hsp70 binding has been localized to TPR1, and Hsp90 binding has been localized to TPR2a. Importantly, the highly conserved. EEVD sequence that terminates many Hsp70 family members, and the similar .MEEVD sequence that terminates Hsp90, are important recognition sites for the TPR domains. The co-crystal structures for TPR1 plus a GPTIEEVD octapeptide (Hsp70 sequence) and TPR2a plus the MEEVD pentapeptide (Hsp90) have been solved (15). HOP is closely related to a human 63kD protein that is sensitive to simian virus SV40 transformation (16) and is related to the yeast heat shock-responsive STI1 gene product (17).\n\nSource:\nRecombinant human HOP protein expressed in E. coli.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 50ng, colorimetric. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H7021-10

Size

50ug

Form

Supplied as a liquid in PBS.

Purity

(same/more than) 90% (SDS-PAGE)

References

1. Chen, S. et al.; J. Biol. Chem. 273: 35194-35200 (1998). 2. Dittmar, K.D. et al.; J. Biol. Chem. 271: 12833-12839 (1996). 3. Kosano, H. et al.; J. Biol. Chem. 273: 32973-32979 (1998). 4. Smith, D.F. et al.; Mol. Cell. Biol. 13: 869-876 (1993). 5. Catelli, M.G. et al;. EMBO J. 4: 3131-3135 (1985). 6. Schuh, S. et al.; J. Biol. Chem. 260: 14292-14296 (1985). 7. Sanchez, E.R. et al.; J. Biol. Chem. 260: 12398-12401 (1988). 8. Beckmann, R.P. et al.; Science 248: 850-854 (1990). 9. Chappell, T.G. et al.; Cell 45: 3-12 (1986). 10. Rothman, J.E.; Cell 59: 591-601 (1989). 11. Chirico, W.J. et al.; Nature (London) 332: 805-810 (1988). 12. Deshaies, R.J. et al.; Nature (London) 332: 8000-805 (1988). 13. Zimmerman, R.M. et al.; EMBO J. 7: 2875-2880 (1988). 14. Ang, D.K. et al.; J. Biol. Chem. 266: 24233-24236 (1991). 15. Scheufler, C. et al.; Cell 101: 199-210 (2000). 16. HonorE?, B.H. et al.; J. Biol. Chem. 267: 8485-8491 (1992). 17. Nicolet, C.M. et al.; Mol. Cell. Biol. 9: 3638-3646 (1989).