HOP, Recombinant Human, 1-543aa, His-Tag (Stress-induced-phosphoprotein 1)

Supplier:

United States Biological

Cat no: H6700-02

HOP, Recombinant Human, 1-543aa, His-Tag (Stress-induced-phosphoprotein 1)

Stress-induced-phosphoprotein1 (STI1), also known as HOP, belongs to the large group of co-chaperones. The main function of STI1 is to link Hsp70 and Hsp90 together. But recent investigations indicate that it also modulates the chaperone activities of the linked proteins and possibly interacts with other chaperones and proteins. It forms a complex with HSC70 and HSPCA/HSP-86 and HSPCB/HSP-84, as well as interacting with PACRG. Recombinant human STI1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nRecombinant corresponding to human STI1, his-tagged, expressed in E. coli. \n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH MEQVNELKEK GNKALSVGNI DDALQCYSEA IKLDPHNHVL YSNRSAAYAK KGDYQKAYED GCKTVDLKPDWGKGYSRKAA ALEFLNRFEE AKRTYEEGLK HEANNPQLKE GLQNMEARLA ERKFMNPFNM PNLYQKLESD PRTRTLLSDP TYRELIEQLR\nNKPSDLGTKL QDPRIMTTLS VLLGVDLGSM DEEEEIATPP PPPPPKKETK PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDTALKHYDKAKELDPTNMT YITNQAAVYF EKGDYNKCRE LCEKAIEVGR ENREDYRQIA KAYARIGNSY FKEEKYKDAI HFYNKSLAEH RTPDVLKKCQ\nQAEKILKEQE RLAYINPDLA LEEKNKGNEC FQKGDYPQAM KHYTEAIKRN PKDAKLYSNR AACYTKLLEF QLALKDCEEC IQLEPTFIKGYTRKAAALEA MKDYTKAMDV YQKALDLDSS CKEAADGYQR CMMAQYNRHD SPEDVKRRAM ADPEVQQIMS DPAMRLILEQ MQKDPQALSE\nHLKNPVIAQK IQKLMDVGLI AIR\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

H6700-02

Size

100ug

Reactivities

Hum

Form

Supplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT, 20% glycerol, 1mM EDTA, 0.2mM PMSF.

Purity

~90% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.Johnson BD., et al. (1998) J Biol Chem. 273(6):3679-86. \n2.Van Der Spuy J., et al. (2001) Biochem J. 21:462-469.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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