Hormone Sensitive Lipase, phosphorylated (Ser855) (HSL, Lipase Hormone Sensitive, LHS, LIPE)

Hormone-sensitive lipase (HSL) is a key enzyme in fatty acid mobilization, overall energy \nhomeostasis, and possibly steroidogenesis.It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1).HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the \nadrenals, ovaries, testes, and macrophages (2, 3).HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development.HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis.The HSL protein levels and enzymatic activity in \nITf and STf were reported positively correlated with serum testosterone levels during development (4). \n \nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

H7021-85

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% glycerol, 0.01% sodium \nazide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Holm C, et al. Science 241(4872):1503-6, 1988 2. Li Z, et al. Genomics 24(2):259-65, 1994 \n3. Osuga J, et al. Proc Natl Acad Sci U S A. 97(2):787-92, 2000 4. Kabbaj O, et al Biology of eproduction 65:601

Additional Info

Recognizes human HSL when phosphorylated at Ser855. Species Crossreactivity: Mouse.

Alternative Names

Hormone Sensitive Lipase Testicular Isoform