Host cell proteins (HCP) content analysis, a critical quality control measure, is essential for determining and quantifying the content of HCP in the final product during bioproduct production. Primary techniques for assessing HCP content include immunological methods, notably enzyme-linked immunosorbent assay (ELISA), and mass spectrometry (MS). ELISA can roughly evaluate HCP levels by detecting HCP-specific antigens. Conversely, liquid chromatography-tandem mass spectrometry (LC-MS/MS) provides deta......
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