Hox 10 (Ceh 10 Homeodomain Containing Homolog (C. elegans), CHX10, Homeobox Protein CHX10, MCOP2, MCOPCB3, RET1, Vsx2)

Applications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml. Detects a band of approximately 46kD in mouse and rat retinal tissue lysates. \nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nPositive Control: Rat or mouse retinal tissue lysate.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H7022-32

Size

250ug

Applications

WB

Hosts

Sheep

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, containing 0.08% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Chen CM & Cepko CL Expression of Chx10 and Chx10-1 in the developing chicken retina. Mech Dev 90:293-7 (2000). 2. Ferda Percin E et al. Human microphthalmia associated with mutations in the retinal homeobox gene CHX10. Nat Genet 25:397-401 (2000). 3. Liu IS et al. Developmental expression of a novel murine homeobox gene (Chx10): evidence for roles in determination of the neuroretina and inner nuclear layer. Neuron 13:377-93 (1994).

Additional Info

Recognizes human HOX10. Species Crossreactivity: Mouse, rat and bovine.