5'-G G T G A(N)8^-3'
3'-C C A C T(N)7^-5'
Concentration: 10u/ul
Source: E.coli that carries the cloned hphIR gene from Haemophilus parahaemolyticus store at-20 degrees C
Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37 degrees C
Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25 degrees C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
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