HPLC peptide mapping analysis integrates the separation capabilities of high-performance liquid chromatography (HPLC) with the qualitative and quantitative capabilities of mass spectrometry (MS). Utilizing the LC-MS/MS platform, which combines HPLC with tandem high-resolution mass spectrometry, peptides are effectively separated by HPLC and their mass and charge are measured by MS. This allows for the identification of peptide segments corresponding to each chromatographic peak, forming a characteristic pep
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