HRP Avidin

Cat no: 405103

Supplier: BioLegend

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Description
Additional Information

Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

Catalogue number:	405103
Applications:	ELISA, ELISPOT, Immunohistochemistry, Western Blot
Size:	1 ml
Clone:	Avidin
Dilutions:	Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	The Avidin-HRP solution should be stored undiluted at 4C. Avoid exposure to sodium azide.
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP