HRP Streptavidin

Streptavidin binds to biotin with high affinity. Streptavidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

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SPECIFICATIONS

Catalog Number

405210

Size

1 ml

Applications

ELISA, ELISPOT, IHC, WB

Dilutions

Each lot of this Streptavidin-HRP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:3000 to determine optimal conditions. For ELISPOT analysis, the reagent should be titrated between 1:500 - 1:1500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

This product is guaranteed for six months. The streptavidin-HRP solution should be stored undiluted at 4C. Avoid exposure to sodium azide.

Alternative Names

Streptavidin-Horseradish Peroxidase, SAv-HRP, streptavidin HRP

Regulatory Status

RUO