B-Bridge HS Master Mix BLUE is a ready to use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. HS DNA Polymerase, NH4 buffer system, dNTPs and magnesium chloride are present in HS Master Mix with Buffer I. Each reaction requires 25 uL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL. HS DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. HS Master Mix offers several advantages. Direct gel loading, no need to use separate loading dyes for electrophoresis and subsequent visualization. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
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