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HS Taq Pol, 2x Master Mix II 1.5 mM MgCl2 (final concentration)

Cat no: BE230704


Supplier: B-Bridge International, Inc.
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B-Bridge HS Polymerase Pre-Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. B-Bridge HS Polymerase Pre-Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer II. Each reaction requires 25 uL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL. B-Bridge HS Polymerase Pre-Mix incorporates a modified form of B-BridgeTaq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. B-Bridge HS Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Catalogue number: BE230704
Size: 1,000Rxn

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