HSC70 Interacting Protein (HIP, AAG2, Aging Associated Protein 2, FAM10A1, FLJ27260, HSPABP1, MGC129952, OTTHUMP00000028873, PRO0786, Progesterone Receptor Associated p48 Protein, Putative Tumor Suppressor ST13, Renal Carcinoma Antigen NY REN 33, SNC6, Su

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP90, HSP70), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and three other proteins termed p23, p60 and heat shock cognate protein 70kD (HSC70)-interacting protein (Hip), also known as p48. Hip, along with HSP70 and p60, combine with steroid receptors as transient intermediate complex members.\n\nIn vitro studies have suggested that Hip transiently interacts with progesterone receptor in a cell free system prior to hormone binding competency and dissociates from the receptor complex with the binding of an HSP90:Immunophilin:p23 subcomplex which then results in a hormone-binding-competent receptor complex. While neither its exact function nor mechanism of action have been identified, Hip appears to be an important factor in steroid receptor function. Hip also appears to regulate the function of HSC70 in an ATP hydrolysis and HSP40 dependent fashion.\n\nCellular Localization: Cytoplasmic\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H7651-05

Size

125ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Ch/Bird, Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG

References

This product has been used in:Prapapanich V et al. Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly. Mol Cell Biol 18:944-52 (1998). <PUBMED:9447991> Prapapanich V et al. Molecular cloning of human p48, a transient component of progesterone receptor complexes and an Hsp70-binding protein. Mol Endocrinol 10:420-31 (1996). <PUBMED:8721986>Hohfeld J et al. Hip, a novel cochaperone involved in the eukaryotic Hsc70/Hsp40 reaction cycle. Cell 83:589-98 (1995). <PUBMED:7585962>

Additional Info

Species Crossreactivity: Crossreacts with Human, Rabbit and Chicken. Not yet tested in other species.