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HSC70 Interacting Protein (ST13/HIP) (AAG2, Aging Associated Protein 2, FAM10A1, FLJ27260, HSPABP1, MGC129952)

Cat no: H7651-07

HSC70 Interacting Protein (ST13/HIP) (AAG2, Aging Associated Protein 2, FAM10A1, FLJ27260, HSPABP1, MGC129952)

ST13/HIP is a cytoplasmic co-chaperone involved in the regulation of Hsc70 chaperone activity and the maturation of progesterone receptor. ST13/HIP is made of two structural and functional domains, an NH2-terminal domain, responsible for the dimerization and the overall asymmetry of the molecule, and a COOH-terminal globular domain, both involved in Hsc70 and unfolded protein binding (1). By collaborating with other positive co-factors such as Hsp40 and the Hsp70-Hsp90 organizing protein (Hop), or competing with negative co-factors such as Bcl2-associated athanogen 1 (Bag1), ST13/HIP facilitates may facilitate the chaperone function of Hsc/Hsp70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and regulators of proliferation or apoptosis (2).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2,500-1:5,000 \nImmunohistochemistry: 1:100-1:250 \nImmunocytochemistry: 1:50-1:100 \nFlow cytometry: 1:20 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

H7651-07

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 50 mM Tris- Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Velten M, et al. J Biol Chem. 277(1):259-66, 2002 2. Shi ZZ, et al. J Zhejiang Univ Sci B. 8(3):170-6, 2007

Additional Info

Recognizes human ST13/HIP. Species crossreactivity: mouse

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