HSL, phosphorylated, Ser853 (LIPE, Hormone-sensitive lipase)

HSL (Hormone-sensitive lipase) is a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis. It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1). HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the adrenals, ovaries, testes, and macrophages (2, 3). HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development. HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis. The HSL protein levels and enzymatic activity in ITf and STf were reported positively correlated with serum testosterone levels during development (4).LIPE, Hormone-sensitive lipase\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50,000-200,000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nWestern Blot: Human skeletal muscle lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

H7021-86

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM tris-glycine, pH7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

References

1. Holm C, et al. Science 241(4872):1503-6, 1988 2. Li Z, et al. Genomics 24(2):259-65, 1994 3. Osuga J, et al. Proc Natl Acad Sci U S A. 97(2):787-92, 2000 4. Kabbaj O, et al Biology of Reproduction 65:601-612, 2001.

Additional Info

Recognizes human HSL at~83kD when phosphorylated at Ser853. Species Crossreactivity: mouse, rat.