Hsp27, phosphorylated (Ser82)

Human Hsp27 belongs to the phylogenically conserved small heat shock protein (smHsp) family, which includes mouse Hsp25, alphaA-crystallins and alphaB-crystallins. Members of this protein superfamily from the animal, plant and microbiotic kingdoms share the so-called alpha-crystallin domain of approximately 90 amino acid residues which is bounded by variable N-and C-terminal extensions \n(1). Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Hsp27 is believed to exist mainly as oligomers of as many as 8

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SPECIFICATIONS

Catalog Number

H1830-52G

Size

50ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Bov, Can, Ch/Bird, GP, Sheep

Form

Supplied as a liquid, 40% glycerol

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. MacRae, T.H (2000) Cell Mol. Life Sci. 57: 899-913. \n2. Ehrnsperger, M., Graber, S., Gaestel, M. and Buchner, J. (1997) EMBO J. 16: 221-229 \n3. Maizels, E.T., Peters, C.A., Kline, M., Cutler, Jr., R.E., Shanmugam, M. and Hunzicker-\nDunn, M. (1998) Biochem. J. 332: 703-712. \n4. Butt, E., Immler, D., Meyer, H.E., Kotlyarov, A., Laab, K. and Gaestel, M (2001) J. Biol. \nChem. 276: 7108-7113 \n5. Benndorf, R., Hayess, K., Ryazantsev, S., Wieske, M., Behlke, J and Lutsch, G. (1994) J. \nBiol. Chem. 269: 20780-20784 \n6. Mehlen, P., Hickey, E., Weber, L. A. and Arrigo, A-P. (1997) Biochem. Biophys. Res. \nCommun. 241:187-192 \n

Additional Info

Detects an ~27kD protein, corresponding to the apparent molecular mass of phosphorylated Hsp27 (Ser82) on SDS-PAGE immunoblots. Species crossreactivity: human, bovine, canine, chicken, guinea pig and sheep. This antibody is specific for phosphorylated Hsp27 (Ser82) and does not exhibit reactivity with non-phosphorylated Hsp27. Specificity for phospho-Hsp27 was demonstrated in peptide competition studies with phosphorylated and non-phosphorylated peptides